Abstract: This report is to our knowledge the first to study plant growth promotion and biocontrol characteristics of Bacillus isolates from extreme environments of Eastern Algeria. Seven isolates of 14 (50 %) were screened for their ability to inhibit growth of some phytopathogenic fungi on PDA and some roots exudates. The bacteria identification based on 16S r-RNA and gyrase-A gene sequence analysis showed that 71 % of the screened isolates belonged to Bacillus amyloliquefaciens and the rest were closely related to B. atrophaeus and B. mojavensis. Most of them had high spore yields (22?×?108–27?×?108 spores/ml). They produced protease and cellulase cell wall-degrading enzymes while the chitinase activity was only observed in the B. atrophaeus (6SEL). A wide variety of lipopeptides homologous was detected by liquid chromatography–electrospray ionization–mass spectrometry analysis. Interestingly, some additional peaks with new masses were characterized, which may correspond to new fengycin classes. The isolates produced siderophores and indole-3- acetic acid phytohormone. The greenhouse experiment using a naturally infested soil with Sclerotonia sclerotiorum showed that the B. atrophaeus (6SEL) significantly increased the size of the chickpea plants and reduced the stem rot disease (P?
N° Revue: Applied biochemistry and biotechnology, 172, 4 - Pagination: 1735-174 - Date: 2014/2/1
URL: http://link.springer.com/article/10.1007/s12010-013-0617-0
Mots cles: Extreme environmentsBacillusBiocontrolPlant growth promotionLipopeptidesIAASiderophores
co_auteurs: Asma Ait-Kaki, Noreddine Kacem-Chaouche, Marc Ongena, Mounira Kara-Ali, Laid Dehimat, Karima Kahlat, Philippe Thona
citations: https://scholar.google.fr/scholar?oi=bibs&hl=fr&cites=13671750449141432459&as_sdt=5